Oocyte cryopreservation: clinical outcome of slow-cooling protocols differing in sucrose concentration
نویسندگان
چکیده
منابع مشابه
Clinical outcome of oocyte cryopreservation after slow cooling with a protocol utilizing a high sucrose concentration.
BACKGROUND Recently, interest in oocyte cryopreservation has steadily increased. Newly developed protocols have dramatically improved survival rates, removing perhaps the major hurdle that has prevented this approach from becoming a fully established form of treatment. However, the clinical efficiency of these protocols has not been exhaustively explored and therefore remains controversial. M...
متن کاملSucrose concentration influences the rate of human oocytes with normal spindle and chromosome configurations after slow-cooling cryopreservation.
BACKGROUND Recently described slow-cooling cryopreservation protocols involving elevated sucrose concentration have improved survival frequencies of human oocytes, potentially overcoming a major hurdle that has limited the adoption of oocyte storage. Because implantation rates of embryos from frozen oocytes remain generally low, it is still debated whether, irrespective of survival rates, this ...
متن کاملUltrastructure of human mature oocytes after slow cooling cryopreservation using different sucrose concentrations.
BACKGROUND We studied the ultrastructural characteristics of human mature oocytes frozen/thawed (F/T) using different concentrations of sucrose. Fresh human mature oocytes were used as controls. METHODS The oocytes (n = 48) were fixed in 1.5% glutaraldehyde at sampling (n = 16) or after freeze/thawing performed using a slow cooling method with propane-1,2-diol 1.5 mol/l and sucrose at either ...
متن کاملFreezing within 2 h from oocyte retrieval increases the efficiency of human oocyte cryopreservation when using a slow freezing/rapid thawing protocol with high sucrose concentration.
BACKGROUND A number of factors influence the success of oocyte cryopreservation and subsequent ICSI. The aim of the present study is to establish the ideal time, after oocyte retrieval, for human oocyte cryopreservation via slow freezing/rapid thawing protocol with 0.3 M sucrose concentration in cryoprotectant solution (SF/RT 0.3 M). METHODS Retrospective study with 75 patients on the clinica...
متن کاملCryopreservation of the ovary by vitrification as an alternative to slow-cooling protocols.
OBJECTIVE To study the thermal properties of a cryoprotectant solution, called VS4, and of VS4-impregnated whole sheep ovaries with pedicle. DESIGN Physical and experimental animal study. SETTING Academic research environment. ANIMAL(S) Five- to 6-month-old ewes. INTERVENTION(S) Thermal properties on cooling of a cryoprotectant solution called VS4 were measured by differential scanning ...
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ژورنال
عنوان ژورنال: Reproductive BioMedicine Online
سال: 2007
ISSN: 1472-6483
DOI: 10.1016/s1472-6483(10)60764-x